JRI 

Establishment of new murine embryonic stem cell lines from Balb/c strain

Vol. 4, Issue 2, / April-June 2003
(pages 96-105)

Hossein Baharvand Corresponding Author
- Embryology Department, Royan Institute, Iranian Academic Center for Education, Culture & Research (ACECR), Tehran, Iran
Klass Matthaei
- Gene targeting Lab, John Curtin School of Medical Research, The Australian National University, Canberra, Australia

Received: 4/1/2003 Accepted: 4/1/2003 - Publisher : Avicenna Research Institute

Related Articles
in Google Scholar
in PubMed

 

Other Format
pdfFull Text (En)
pdfFull Text (Fa)
pdfAbstract (En)
pdfAbstract (Fa)
pdfePUB (En)
pdfBibTeX
pdfRefMan
pdfEndNote
xmlPMC XML
online readerPMC Reader
online readerHBIs XML

 


Abstract

Embryonic stem cells are pluripotent cells with self renewal ability that are derived from inner cell mass of blastocysts. It is possible to differentiate the cells under appropriate culture conditions into specific cells. Moreover, manipulation of their genome in-vitro allows the creation of transgenic and knockout mice. Owing to the significance of ES cells, this study was conducted to produce new lines of mice-blastocysts aged 3.5 days were recovered from BALB/c mouse strain and cultured on mouse embryonic fibroblasts in ES media supplemented with 1000IU/ml or 5000IU/ml leukemia inhibitory factor (LIF). Established lines were analyzed for simple karyotype, C-banding, polymerase chain reaction of SRY gene (PCR-SRY), alkaline phosphates, and Oct-4 expression (RT-PCR). Three ES cell lines were produced morphologically. These lines were isolated in 5000 IU/ml LIF only. All three lines were male. Two lines had normal karyotype (40 chromosomes) and one line was tetraploid. C-banding and SRY-PCR showed that all lines had XY sex chromosome composition. Also, all lines had alkaline phosphates activity and expressed Oct-4. These results indicated that two male murine ES cell lines with normal karyotype, alkaline phosphates and Oct-4 positive properties were established from BALB/c strain.


Keywords: Embryonic stem cell, Mouse, Balb/c strain, Pluripotency


To cite this article:


References

  1. Evans M.J., Kaufman M.H. Establishment in culture of pluripotential cells from mouse embryos. Nature. 1981; 292,154-156.
  2. Martin G.R. Isolation of apluripotent cell line from early mouse embryos culture in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci USA. 1981;78,7634-7638.
  3. Brook F.A., Gardner R.L. The origin and efficient derivation of embryonic stem cells in the mouse. Proc Natl Aced Sci SA.1997;94,5709-5712.
  4. Hong Y.C؛ Winkler M. Schartl Production of medakafish chimeras from a stable embryonic stem cell line.Proc Natl Acad Sci USA. 1998;95:3679-3684.
  5. Sun L.C.S., Bradford G., Ghosh. PCollodi: ES-like cell cultures derived from early zebra fish embryos.Mol Mar Boil Biotechnol. 1995;4:193-199.
  6. Chang I.K., D.L. Jeong., Y.H. Hong, T.S. Park, Y.K. Moon, T. Ohon, J.Y. Han. Production of germ line chimeric chickens by transfer of cultured primordial germ cells. Cell Biol Int. 1997; 21:495-499.
  7. Schoonjans L., GM Albricht., JL Li., D Collen., RW Moreadith. Pluripotential rabbitembryonic stem (ES) cells are capable of forming overcoat color chimeras following injection into blastocysts. Mol Reproed Dev. 1996; 45:439-443.
  8. Moens A.B., Flechon J., Degrouard X., Vignon J., Ding J.E., Flechon K.J; Betteridge J.P. Renard. Ultra structural and immunocytochemical analysis of diploid germ cells isolated from fetal rabbit gonads. Zygote. 1997;5:47-60.
  9. Jannaccone P.M. Pluripotent embryonic stem cells from the rat are capable of producing chimeras. Dev Biol. 1994;163: 288-292.
  10. Doetschman T., Williams C.P., Maeda M. Establishment of hamster blastocyst-derived embryonic stem (ES) cells. Dev Biol. 1988;127:224-227.
  11. Wheeler M.B. Development and validation of swine embryonic stem cells. A review. Reprod Fertil Dev. 1994;6:563-568.
  12. Piedrahita J.A., Moore K., Octama B., Lee C.K., Seales N., Ramsoondar J., Baze F.W. Ott T.Generation of transgenic porcine chimeras using primordial germ cell-derived colonies. Biol Reprod. 1998; 58:1321-1329.
  13. Cibelli J.B., Stice S.L., Golueke P.G., Kane., J Jerry J.J., Blackwell E.S.C., Ponce de Leon F.A., Robl M.J. Transgenic bovine chimeric offspring produced from somatic cell-derived stem-likecells. Nat Biotechnol. 1998;16:642-646.
  14. Wells O.N., Misica P.M., T.A. Day M., Tervit H.R. Production of cloned lambs from an stem (ES) cell lines. Int J Dev Biol. 1994;38:385-390.established embryonic cell line: A comparison between in vivo and in vitro matured cytoplasts. Biol Reprod. 1997;57:385-393.
  15. Thomson J.A., Kalishman J., Golos T.G., Durning M., Harris CP., Becker RA., Hearn J. Isolation of a primate embryonic stem cell line. Proc. 1995;92:7844-7848.
  16. Thompson J.A. Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J. Embryonic stem cell lines derived from human blastocysts. Science. 1998;282: 1145-1147.
  17. Soria B., Roche E., Berna G., Leon-Quinto T., Reig J.A., Martin F. Insulin-secreting cells derived from embryonic stem cells normalizeglycemia in streptozotocin-induceddiabetic mice. Diabetes. 2000;49,157162.
  18. Arenas E. Stem cells in the treatment of Parkinson’s disease. Brain Res Bull. 2002;57(6):795-808.Review.
  19. Cantin E., Mann J. Genetic variation among 129 sub strains. Practical consequences. J Immunol. 1999;162: 6294-5295.
  20. Sechler J.M., Yip J.C., Rosenberg A.S. Genetic variation among 129 substrains: practical consequences.J Immunol. 1997; 159:5766-5768.
  21. Simpson E.M., Linder C,C., Sargent E.E., Davisson M.T., Mobraaten L.E., Sharp J.J. Genetics variation among 129 sub strains and its importance for targeted mutagenesis in mice. Nat1 Genet. 1997; 16:19-27.
  22. Abbondanzo S.J., Gadi I., Stewart C.L. Derivation of embryonic stem cellines. Methods in Enzymology, Wasserman, PM Depamphilis M.L., Eds. Academic Press, San Diego. 1993;225, 803-823.
  23. Robertson E.J. Derivation and maintanance of embryonic stem cell cultures. Methods Mol Biol. 1997;75: 173-184.
  24. Hogan B., Constantini F., Lacy E. Manipulating the mouse embryo. A laboratory manual. Cold Spring Harbor laboratorial. Cold Spring Harbor, NY. 1994.
  25. Roach M.L., McNeish J.D. Methods for the isolation and maintenance of murineembryonicstem cells. Methods in Molecular Biology. 2002;1850:1-16.
  26. حسين بهاروند، ‌كلاس ماتايي،‌ كاظم پريور،‌ توليد رده‌هاي جديد سلولهاي بنيادي جنيني از موش نژاد C57BL/6،‌ مجله علمي پژوهشي كوثر، ‌در دست چاپ.
  27. Nichols J., Zevnik B., Anastassiadis K., Niwa H., Klewe-Nebenius D., Chambers I., Scholer H., Smith A. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct 4.Cell. 1998;5:379-391.
  28. Smith A.G. embryo-derived stem cells of mice and men. Annu Rev Cell Dev Biol. 2001;17:435-462.
  29. Robertson E., Bradley A., KaehnM., Evans M. Germ-line transmissionof genes introduced into cultured pluripotential cells by retrovial vector. Nature. 1986;323: 445-448.
  30. Gossler A., Doetschman T., Korn R., Serfling E., Kemler R9. Transgenesis by means of blastocyst-derived embryonic stem cell lines. Proc Natl Acad Sci USA. 1986;83:9065-9069.
  31. Hooper M., Hardy K., Handyside A., Hunter S., Monk M. HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germ line colonization by cultured cells. Nature. 1987;326,292-295.
  32. McMahon A.P., Bradley A. The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain. Cell. 1990;62:1073-1085.
  33. Nichols J., Evans E.P., Smith A.G. Establishment of germ-line competent embryonic stem (ES) cells using differentiation inhibiting activity. Development. 1990;110:1341-1348.
  34. Tokunaga T., Tsunoda Y. Efficacious production of viable germ-line chimerase between embryonic stem (ES) cells and 8-cell stage embryos. Dev Growth Differ. 1992;34:561-566.
  35. Yagi T., Tokunaga T., Furuta Y., Nada S., Yoshida M. A novel ES cell line, TT2, with high germ line. Differentiating potency. Anat Bioch. 1993;214,70-76.
  36. Ledermann B., Burki K. Establishment of a germ-line competent C57BL/6 embryonic stem line. Exp Cell Res. 1991;197:254-258.
  37. Nagafuchi S., Katsuta H., Kogawa K., Akashi T. Establishment of and embryonic stem (ES) cell line derived from a non obese diabetic (NOD)mouse: in vitro differentiation into lymphocytes and potential for germ line transmission. FEBS Letters. 1999;455,101-104.
  38. Kawase E., Saemori H., Takahashi N., Okazaki K., Hashimoto K., Nakatsuji N. Strain difference in establishment of mouse embryonic stem (ES) cell lines. Int J Dev Biol. 1994;38:385-390.
  39. Stevens L.C. The development of transplantable teratocarcinomas from intratesticular grafts of pre and post implantation mouse embryos. Dev Biol. 1970;21,364-382.
  40. Nichols J., Evans E., Smith A.G. Establishment of germ-line-competent embryonic stem (ES) cell sousing differentiation inhibiting activity. Development. 1990;110:1341-1348.
  41. Rastan S., Robertson E.J. X-chromosome deletions in embryo-derived (EK) cell line associated with lack of X-chromosome inactivation. J Embryol Exp Morphol. 1985;90:379-388.
  42. Bradley A., Evans M.J., Kaufman M.H. Robertson E. Formation of germ-line chimaeras from embryo-derived teratocar cinoma cell lines. Nature. 1984;309:255-256.
  43. Pain B., ME Clark M., Shen., H.N akazawa M., Sakura J., Samarut. Etches R.J. Long term in vitro culture and characterizations of avian embryonic stem cells with multiple morphogenic potentialities. Development. 1996;122: 2339-2348.

COPE
SID
NLM
AJMB
IJBMLE
IJBMLE

Home | About Us | Current Issue | Past Issues | Submit a Manuscript | Instructions for Authors | Subscribe | Search | Contact Us

"Journal of Reproduction & Infertility" is owned, published, and managed by Avicenna Research Institute .
Creative Commons License

This work is licensed under a Creative Commons Attribution –NonCommercial 4.0 International License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.

Journal of Reproductoin and Infertility (JRI) is a member of COMMITTEE ON PUBLICATION ETHICS . Verify here .

©2024 - eISSN : 2251-676X, ISSN : 2228-5482, For any comments and questions please contact us.