- Jafar Ai
 - 1- Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
- 2- Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran
- Navid Esfandiari
- - Division of Reproductive Sciences, Department of Obstetrics and Gynecology, Toronto Centre for Advanced Reproductive Technology (TCART),University of Toronto, Samuel Lunenfeld Research Institute, Toronto, Canada
- Robert Casper
- - Division of Reproductive Sciences, Department of Obstetrics and Gynecology, Toronto Centre for Advanced Reproductive Technology (TCART),University of Toronto, Samuel Lunenfeld Research Institute, Toronto, Canada
Received: 12/30/2008 Accepted: 5/9/2009 - Publisher : Avicenna Research Institute |
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Abstract
Introduction: Endometriosis is the presence of endometrial glandular and stromal cells outside the uterine cavity. The disease is prevalent in about 10% of women of reproductive age and in up to 50% of infertile women. Surgery continues to be the first line of treatment in eradicating endometriotic lesions but recurrence of the condition is seen in up to 47% of the cases. VEGF is an effective factor in the establishment of endometriosis. The aim of the present study was to determine VEGF levels in a three-dimensional (3D) fibrin matrix culture of human endometrial tissue.Materials and Methods: Endometrial biopsies were obtained from the uterine fundus of 10 ovulating (at 19th to 24th day of menstruation cycle) premenopausal women attending the Toronto Center for Advanced Reproductive Technology (TCART) for the treatment of infertility, ovarian cysts or non-endometrial complaints. Each tissue fragment was divided into ten segments for culture in a total of 100 wells. The samples were cultured on three-dimensional fibrin matrices and the supernatant fluid was collected from each endometrial sample for the assessment of VEGF levels. The endometrial samples were stained by anti-Cox-2 antibody (anti-cyclooxygenase -2) for immunohistochemical evaluation of angiogenesis.Results: VEGF levels in the supernatant fluid of wells with angiogenesis was significantly higher (p<0.05 ) than wells with no signs of the phenomenon (492±3.11 and 183±13.2, respectively). the results were indicative of cell proliferation in 91% of the wells and angiogenesis was observed in 51 wells (56%) with cell proliferation.conclusion: it seems that vegf secretion plays an important role in promoting neovasculariza-tion and cell proliferation of endometrial cells. therefore, vegf secretion seems to be involved in the establishment of endometriosis. seeking the relationship between cell proliferation and vegf secretion rates is suggested.< pan>
Keywords:
Angiogenesis, Endometriosis, Endometrium, Female infertility, Three-dimensional tissue culture, VEGF To cite this article:References
- Strathy JH, Molgaard CA, Coulam CB, Melton LJ 3rd. Endometriosis and infertility: a laparoscopic study of endometriosis among fertile and infertile women. Fertil Steril. 1982;38(6):667-72.
- Adamson GD, Nelson HP. Surgical treatment of endometriosis. Obstet Gynecol Clin North Am. 1997; 24(2):375-409. Review. [PubMed]
- Kettel LM, Hummel WP. Modern medical management of endometriosis. Obstet Gynecol Clin North Am. 1997;24(2):361-73. Review. [PubMed]
- Rogers PA, Abberton KM, Susil B. Endothelial cell migratory signal produced by human endometrium during the menstrual cycle. Hum Reprod. 1992;7(8): 1061-6. [PubMed]
- Lebovic DI, Bentzien F, Chao VA, Garrett EN, Meng YG, Taylor RN. Induction of an angiogenic phenotype in endometriotic stromal cell cultures by interleukin-1beta. Mol Hum Reprod. 2000;6(3):269-75. [PubMed]
- Fasciani A, Bocci G, Xu J, Bielecki R, Greenblatt E,Leyland N, et al. Three-dimensional in vitro culture of endometrial explants mimics the early stages of endometriosis. Fertil Steril. 2003;80(5):1137-43. [PubMed]
- Halme J, Hammond MG, Hulka JF, Raj SG, Talbert LM. Retrograde menstruation in healthy women and in patients with endometriosis. Obstet Gynecol. 1984;64 (2):151-4. [PubMed]
- Oral E, Arici A. Pathogenesis of endometriosis. Obstet Gynecol Clin North Am. 1997;24(2):219-33. Review. [PubMed]
- Lamb K, Hoffmann RG, Nichols TR. Family trait analysis: a case-control study of 43 women with endometriosis and their best friends. Am J Obstet Gynecol. 1986;154(3):596-601. [PubMed]
- Esfandiari N, Ai J, Nazemian Z, Javed MH, Gotlieb L, Casper RF. Expression of glycodelin and cyclo-oxygenase-2 in human endometrial tissue following three-dimensional culture. Am J Reprod Immunol. 2007;57(1):49-54. [PubMed]
- Esfandiari N, Khazaei M, Ai J, Bielecki R, Gotlieb L, Ryan E, et al. Effect of a statin on an in vitro model of endometriosis. Fertil Steril. 2007;87(2):257-62. [PubMed]
- Becker CM, Bartley J, Mechsner S, Ebert AD. [Angiogenesis and endometriose] Zentralbl Gynakol. 2004;126(4):252-8. German. [PubMed]
- Ferrara N, Davis-Smyth T. The biology of vascular endothelial growth factor. Endocr Rev. 1997;18(1):4-25. Review. [PubMed]
- Kieser A, Weich HA, Brandner G, Marmé D, Kolch W. Mutant p53 potentiates protein kinase C induction of vascular endothelial growth factor expression. Oncogene. 1994;9(3):963-9. [PubMed]
- Yoo SA, Kwok SK, Kim WU. Proinflammatory role of vascular endothelial growth factor in the pathogenesis of rheumatoid arthritis: prospects for therapeutic intervention. Mediators Inflamm. 2008; 2008:129873. Review. [PubMed]
- Lieb W, Safa R, Benjamin EJ, Xanthakis V, Yin X, Sullivan LM, et al. Vascular endothelial growth factor, its soluble receptor, and hepatocyte growth factor: clinical and genetic correlates and association with vascular function. Eur Heart J. 2009;30(9):1121-7. [PubMed]
- Donnez J, Smoes P, Gillerot S, Casanas-Roux F, Nisolle M. Vascular endothelial growth factor (VEGF) in endometriosis. Hum Reprod. 1998;13(6):1686-90. [PubMed]
- Rogers PA, Donoghue JF, Walter LM, Girling JE. Endometrial angiogenesis, vascular maturation, and lymphangiogenesis. Reprod Sci. 2009;16(2):147-51. [PubMed]
- Cosin R, Gilabert-Estelles J, Ramon LA, Espana F, Gilabert J, Romeu A, et al. Vascular endothelial growth factor polymorphisms (-460C/T, +405G/C, and 936C/T) and endometriosis: their influence on vascular endothelial growth factor expression. Fertil Steril. 2008 Oct 16. [Epub ahead of print]. [PubMed]
- Godo G, Sas M, Falkay G. Bromocryptine therapy in luteal insufficiency. Acta Med Acad Sci Hung. 1980;37 (3):283-8. [PubMed]
- Cunha-Filho JS, Gross JL, Lemos NA, Brandelli A, Castillos M, Passos EP. Hyperprolactinemia and luteal insufficiency in infertile patients with mild and minimal endometriosis. Horm Metab Res. 2001;33(4): 216-20.
- Ferté C, Massard C, Moldovan C, Desruennes E, Loriot Y, Soria JC. Wound healing delay after central venous access following DCF/VEGF-trap therapy. Invest New Drugs. 2009 Feb 17. [Epub ahead of print]. [PubMed]
- Esfandiari N, Ai J, Khazaei M, Nazemian Z, Jolly A, Casper RF .Angiogenesis following three-dimensional culture of isolated human endometrial stromal cells. Int J Fertil Steril. 2008;2(1):19-22. [Abstract]
- Fujii EY, Nakayama M, Nakagawa A. Concentra-tions of receptor for advanced glycation end products, VEGF and CML in plasma, follicular fluid, and peritoneal fluid in women with and without endometriosis. Reprod Sci. 2008;15(10):1066-74. [PubMed]