Association of ACE, PAI-1 and coagulation factor XIII gene polymorphisms with recurrent spontaneous abortion in Iranian patients

Soltanghoraee, Haleh; Memariani, Taktom; Aarabi, Mahmood; Hantoushzadeh, Sedigheh; Arefi, Soheila; Aarabi, Mohsen; Akhondi, Mohammad Mehdi; Modarresi, Mohammad Hossein

Association of ACE, PAI-1 and coagulation factor XIII gene polymorphisms with recurrent spontaneous abortion in Iranian patients

Vol. 7, Issue 4, / January-March 2007
(pages 324-330)

Haleh Soltanghoraee: 1- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran; 2- Avicenna Infertility Clinic, Avicenna Research Institute (ACECR), Tehran, Iran
Taktom Memariani: - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Mahmood Aarabi: - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Sedigheh Hantoushzadeh: - Faculty of Medical Tehran University of Medical Sciences, Tehran, Iran
Soheila Arefi: 1- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran; 2- Avicenna Infertility Clinic, Avicenna Research Institute (ACECR), Tehran, Iran
Mohsen Aarabi: - Academic Unit of clinical Pharmacology ,University of Sheffield, Sheffield, United Kingdom
Mohammad Mehdi Akhondi: - Monoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran
Mohammad Hossein Modarresi: 1- Faculty of Medical Tehran University of Medical Sciences, Tehran, Iran; 2- Nanobiotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran

Received: 1/1/2007 Accepted: 1/1/2007 - Publisher : Avicenna Research Institute

Other Format

Abstract

Introduction: Considering the 5% prevalence of abortion in women, its devastating psycholo-gical effects on affected people’s lives and as tendency for clotting is one of the causes of recur-rent spontaneous abortion (RSA), this study was designed to compare plasminogen activator inhibitor-1 (PAI-1), angiotensin converting enzyme (ACE) and blood coagulation factor XIII (FXIII) gene polymorphisms in Iranian patients with RSA and healthy women. Materials & Methods: 120 patients with recurrent abortions (at least two) as cases and 112 healthy female controls without a history of abortion were entered into the study. In order to characterize PAI-1 (4G/5G), ACE (D/I) and FXIII (Val 34 Leu) polymorphisms, a polymerase chain reaction followed by digestion with restriction enzymes (PCR-RFLP) was designed. For the statistical analysis, SPSS software version 11.2 was used and t-test, Chi-square and Fisher’s exact tests were calculated. P-values<0.05 were considered as significant. results: homozygosity for pai-1 4g polymorphism was seen in 16 cases, (14.4%), in contrast to two persons in the control group (2%), (p="0.001)" and patients with homozygote 4g mutation were significantly more prone to rsa in contrast to others (or: 8.2, % 95 ci: 1.8- 36.5). 38 patients, (29.5 %), and 25 people from the control group, (26.6%), were homozygote (d ) for ace polymorphism, depicting no statistically significant difference. only two patients and one person from the control group had homozygosity (34leu) for fxiii polymorphism. conclusion: 4g g polymorphism for pai-1 gene could be a thrombophilic mutation leading to abortion. analysis of this mutation and other suspected factors such as mthfr and fv leiden is recommended in patients with rsa. in this study, there were no significant associations between ace and fxiii with rsa.< pan>

Keywords: Recurrent spontaneous abortion, Thrombophilia, Plasminogen activator inhibitor-1, Angiotensin converting enzyme, Coagulation factor XIII

To cite this article:

Soltanghoraee H, Memariani T, Aarabi M, Hantoushzadeh S, Arefi S, Aarabi M, et al. Association of ACE, PAI-1 and coagulation factor XIII gene polymorphisms with recurrent spontaneous abortion in Iranian patients. J Reprod Infertil. 2007;7(4):324-330.

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