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The effects of Human Chorionic Gonadotropin on germ cell maturation and testosterone secretion in neonatal mouse testis

Vol. 7, Issue 3, / October-December 2006
(pages 209-216)

Reza Akbarzadeh Najar
1- Department of Biochemistry, Faculty of Sciences, Islamic Azad University, Science & Research Campus, Tehran, Iran
2- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Mohammad Mehdi Akhondi Corresponding Author
- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Kazem Parivar
- Department of Biology, Faculty of Sciences, Islamic Azad University, Science & Research Campus, Tehran, Iran
Mahmood Jeddi-Tehrani
- Monoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran
Mohammad Reza Sadeghi
1- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
2- Monoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran
Ebrahim Djavadi
- Endocrinology and Metabolism Research Center (EMRC), Tehran University of Medical Sciences, Tehran, Iran

Received: 10/1/2006 Accepted: 10/1/2006 - Publisher : Avicenna Research Institute

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Abstract

Introduction: Human chorionic gonadotropin (hCG) as an LH agonist affects spermatogenesis and germinal cell numbers, and has extensive usages in infertility treatments. The aim of this study was to determine the effects of varied doses of hCG on germinal cell proliferation and androgenic status in mouse model.Materials & Methods: In this study, hCG dosages of 5 to 50 IU were injected into 18 mice in three experimental groups and 6 mice served as the control group (Group 1). The mice in groups 2, 3 and 4 received subcutaneous injections of 5, 10 and 50 IU doses of hCG respectively, on days 15 and 25 of their lives. Blood samples were obtained from each mouse on days 28 and 65 for serum measurements of testosterone. One testis of each mouse was harvested for flow cytometric DNA analysis on day 65. Results: Serum testosterone levels on day 28 were greater in groups 2, 3 and 4 compared to that of the control group. With increasing doses of hCG, the mean testosterone levels increased too and the highest values were observed in group 4. However, serum testosterone levels on day 65 were greatest in group 1 but progressively decreased in groups 2, 3 and 4, lowest in group 4, but there were no significant statistical differences among the groups. Groups 3 and 4 had a signi-ficantly reduced mean haploid cell numbers on day 65.Conclusion: The results of this study showed that testosterone production in neonatal mouse testis increases after hCG injection and there is a linear relationship between serum testosterone and hCG injections. With the passage of time and clearance of hCG, Leydig cell stimulation decreases and subsequently testosterone levels diminish too, especially in mice with highest doses of hCG injections. Therefore, for testosterone production in neonatal mouse testis, conti-nuous stimulation of Leydig cells is essential.


Keywords: HCG, Apoptosis, Flow cytometry, Testosterone, Testis, Hormone, Germ cells


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References

  1. Berkowitz R, Ozturk M, Goldstein D, Bernstein M, Hill L, Wands JR. Human chorionic gonadotropin and free subunits' serum levels in patients with partial and complete hydatidiform moles. Obstet Gynecol. 1989; 74(2):212-6.   [PubMed]
  2. Ozturk M, Bellet D, Manil L, Hennen G, Frydman R,Wands J. Physiological studies of human chorionic gonadotropin (hCG), alpha hCG, and beta hCG as measured by specific monoclonal immunoradiometric assays. Endocrinolgy. 1987;120(2):549-58.   [PubMed]
  3. Al-Hamood MH, Gilmore DP, Wilson CA. Evidence for a stimulatory beta-adrenergic component of the preovulatory LH surge in proestrus rats. J Endocrinol. 1985;106(2):143-51.   [PubMed]
  4. John Radcliffe. Cryptorchidism: a prospective study of 7500 consecutive male births, 1984-8. Hospital Cryptorchidism Study Group. Arch Dis Child. 1992;67:892-899.   [PubMed]
  5. Hjertkvist M, Lackgren G, Ploen L, Bergh A. Does hCG treatment induce inflammation-like changes in undescended testes in boys?. J Pediatr Surg. 1993;28(2):254-258.   [PubMed]
  6. Demirbilek S, Atayurt HF, Cilek N, Aydin G. Does treatment with human chorionic gonadotropin induce reversible changes in undescended testes in boys?Pediatr Surg Int. 1997;12(8):591-594.   [PubMed]
  7. Polascik TJ, Chan-Tack KM, Jeffs RD, Gearhart JP. Reappraisal of the role of human chorionic gonadotropin in the diagnosis and treatment of the nonpalpable testis: a 10-year experience. J Urol. 1996;156:804-806.   [PubMed]
  8. Bukowski TP, Sedberry S, Richardson B. Is human chorionic gonadotropin useful for identifying and treating nonpalpable testis? J Urol. 2001;165:221-223.   [PubMed]
  9. Fedder J, Boesen M. Effect of a combined GnRH/ hCG therapy in boys with undescended testicles: evaluated in relation to testicular localization within the first week after birth. Arch Androl. 1998;40:181-186.   [PubMed]
  10. Giannopoulos MF, Vlachakis IG, and Charissis GC.13 years experience with the combined hormonal therapy of cryptorchidism. Horm Res. 2001;55:33-37.   [PubMed]
  11. Tapanainen JS, Tilly JL, Vihkon KK, Hsueh AJ. Hormonal control of apoptotic cell death in the testis:gonadotropins and androgens as testicular cell survival factors. Mol Endocrinol. 1993;7:643-650.   [PubMed]
  12. Billig H, Furuta I, Rivier C, Tapanainen J, Parvinen M, Hsueh AJ. Apoptosis in testis germ cells: developmental changes in gonadotropin dependence and localization to selective tubule stages. Endocrinology. 1995;136:5-12.   [PubMed]
  13. Hikim AP, Wang C, Leung A, Swerdloff RS. Involvement of apoptosis in the induction of germ cell degeneration in adult rats after gonadotropin releasing hormone antagonist treatment. Endocrinology. 1995;136:2770-2775.   [PubMed]
  14. Kaleva M, Arsalo A, Louhimo I, Rapola J, Perheentupa J, Henriksen K, Toppari J. Treatment with human chorionic gonadotropin for cryptorchidism: clinical and histological effects. Int J Androl. 1996;19:293-298.   [PubMed]
  15. Bergh A, Widmark A, Damber JE, Kajander S. Are leukocytes involved in the human chorionic gonadotropin-induced increase in testicular vascular permeability?Endocrinology. 1986;119:586-590.   [PubMed]
  16. Bergh A, Rooth P, Widmark A, Damber JE. Treatment of rats with hCG induces inflammation-like changes in the testicular microcirculation. J Reprod Fertil. 1987;79:135-143.   [PubMed]
  17. Widmark A, Bergh A, Damber J-E. Leukocytes mediate the hCG induced increase in testicular venular permeability. Mol Cell Endocrinol. 1987;53:25-31.   [PubMed]
  18. Heiskanen P, Billig H, Toppari J, Kaleva M, Arsalo A, Rapola J, Dunkel L. Apoptotic cell death in the normal and cryptorchid human testis: the effect of human chorionic gonadotropin on testicular cell survival. Pediatr Res. 1996;40:351-356.   [PubMed]
  19. Dunkel L, Taskinen S, Hovatta O. Germ cell apoptosis after treatment with human chorionic gonadotropin is associated with impaired reproductive function in the adult. J Clin Invest. 1997;100:2341-2346.   [PubMed]
  20. Taskinen S, Wikstrom S. Effect of age at operation,location of testis and preoperative hormonal treatment on testicular growth after cryptorchidism. J Urol. 1997;158:471-473.   [PubMed]
  21. Chandrasekharam VV, Srinivas M, Das SN, Jha P, Bajpai M, Chaki SP, Misro MM. Prepubertal human chorionic gonadotropin injection affects postpubertal germ cell maturation and androgen production in rat testis. Urology. 2003;62:571-574.   [PubMed]
  22. Troiano L, Fustini MF, Lovato E, Frasoldati A, Malorni W, Capri M, Grassilli E, Marrama P, FranceschiC. Apoptosis and spermatogenesis: evidence from an in vivo model of testosterone withdrawal in the adult rat. Biochem Biophys Res Commun. 1994;202:1315-1321.   [PubMed]
  23. Oakland E. A description of spermatogenesis in the mouse and its use in analysis of the cycle of seminiferous epithelium and germ cell renewal. Am J Anat. 1956;99:391-413.
  24. Huckins C. The morphology and kinetics of spermatogonial degeneration in normal adult rats: an analysis using a simplified classification of germinal epithetlium. Anat Rec. 1978;190:905-926.   [PubMed]
  25. De Rooij DG, Lok D. Regulation of the density of spermatogonia in the seminiferous epithelium of the Chinese hamster: II. Differentiating spermatogonia. Anat Rec. 1987;217:131-136.   [PubMed]
  26. Russel LD, Alger LE, Nequin LG. Hormonal control of pubertal spermatogenesis. Endocrinology. 1987;120:1615-1632.   [PubMed]
  27. Clermont Y, Morgentaler H. Quantitative study of spermatogenesis in the hypophysectomized rats. Endocrinology. 1955;57:369-382.   [PubMed]
  28. Raj LD, Dym M. The effects of selective withdrawal of FSH and LH on spermiogenesis in the immature rat. Biol Reprod. 1976;14:489-494.   [PubMed]
  29. Troiano L, Fustini M, Lovato E, Frasoldati A, Malorni W, Capri M, Grassilli E, Marrama P, Franceschi C. Apoptosis and spermatogenesis: evidence from an in vivo model of testosterone withdrawal in the adult rat. Biochem Biophys Res Commun. 1994;202:1315-1321.   [PubMed]
  30. Dunkel L, Perheentupa J, Apter D. Kinetics of the steroidogenic response to single versus repeated doses of human chorionic gonadotropin in boys in prepuberty and early puberty. Pediatr Res. 1985;19:1-4.   [PubMed]
  31. Veijola M, Kellokumpu S, Rajaniemi H. The effect of varying doses of hCG on the in vivo uptake by rat testis and serum testosterone response. Horm Res.1984;19:191-199.   [PubMed]
  32. Hodgson YM, de Kretser DM. Serum testosterone response to single injection of hCG, ovine-LH and LHRH in male rats. Int J Androl.1982;5:81-91.   [PubMed]
  33. Giwercman A, Clausen OP, Brunn E. The value of quantitative DNA flowcytometry of testicular fineneedle aspirates in assessment of spermatogenesis: a study of 137 previously maldescended human testes. Int J Androl. 1994;17:35-42.   [PubMed]

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