- Jaleh Shojaeian
- - Immunology Department, Faculty of Medical Science, Tarbiat Modarres University, Tehran, Iran
- Seyyed Mohammad Moazzeni
 - - Immunology Department, Faculty of Medical Science, Tarbiat Modarres University, Tehran, Iran
- Amir Hassan Zarnani
- - Monoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran
Received: 7/1/2004 Accepted: 7/1/2004 - Publisher : Avicenna Research Institute |
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Abstract
Introduction: Accumulating evidence suggests that the fetus as an allograft is protected by local and systemic modulation of maternal immune responses. It seems that the presence of inhibitory factor(s) in pregnant sera may thus serve as a mechanism for the modulation of maternal immune responses. In this study the effects of normal and pregnant mouse sera on DC function, that is an antigen presenting cell (APC) with a unique ability to induce primary immune responses, were examined by allogeneic MLR. Materials and Methods: Sera were collected from allogeneic pregnant mice in early, mid and late gestation and were stored in -70oC. DCs were isolated from Balb/C mouse spleens by collagenase digestion and separation of low-density cells using nycodenz gradient. The purity of isolated DCs was determined more than 95% by flow cytometric analysis. Isolated DCs were cultured in RPMI containing early, mid and late pregnant mouse sera. These cells were used as stimulator cells in allogeneic MLR after washing and 3000 Rad irradiation. T cells were also were isolated from C57BL/6 mouse lymph nodes by nylon wool method. The final purity of T cells was determined approximately 90% by flow cytometry. The DCs and T cells were co-cultured in allogeneic MLR and cell proliferation was measured by 3H thymidine incorporation. Results: Results showed that sera of early and late pregnancy didn’t have any effect on DC function, in comparison with normal mouse sera, while mid-pregnancy sera suppressed DC stimulation capacity significantly. Conclusion: To our knowledge, this is the first report of the effect of pregnant serum on DC functions. Reduced MLR response in mid-pregnancy may be due to overflow of the locally present suppressor factors such as IL-4, IL-10, TGF-β and PGE-2.
Keywords:
Dendritic cell, Spleen, Pregnancy, Serum, Mixed leukocyte culture, Mouse To cite this article:References
- Medawar P.B. Some immunological and endocrinological problems raised by the evolution of viviparity in vertebrates.Symp Soc Exp Biol. 1953;7:320-338.
- Thellin O., Heinen E. Pregnancy and the imm-une system: between tolerance and rejection. Toxicology.2003; 185:179-184.
- Mellor A.L., Munn D.H. Immunology at the maternal-fetal interface: lessons for T cell tole-rance and suppression. Annu Rev Immunol.2000;18:367-91.
- مصـفا نـريمان، زرنانـي اميـرحسن، حسن زهيرمحمد. ايمونولوژي حاملگي طبيعي. انتشارات دانشگاه علوم پزشكي و خدمات بهداشتي، درماني شهيد بهشتي (1382)، صفحات: 181-164.
- Heyborne K., Silver R.M. Immunology of postimplantation pregnancy, In: Reproductive Immunology, Boronson R. (Editors), Blackwell scientific publitions Ltd.1996; pp: 383-417.
- Letellier C., Fizet D., Baltz T., Vezon G. Immu-nosuppressive activity of sera of pregnant women on cytotoxic T-lymphocyte-mediated cytolysis. I characterization of the active fraction. Gynecol Obstet Invest.1994; 37:1-5.
- Banchereau J., Briere F., Caux C., Davoust J., Lebecque S., Liu Y.J., Pulendran B., Palucka K. Immunobiology of dendritic cells. Ann Rev Immunol.2000; 18:767-811.
- Bhzrdwaj N. The modulation of immunity by dendritic cells. Clin Appl Immun Rev.2003; 3:173 -82.
- Pulendran B., Banchereau J., Maraskovsky E., Maliszewski C. Modulating the immune response with dendritic cells and their growth factors. Trends Immunol.2001; 22:41-47.
- Sorense A.N., and Tribe D.E. Breeding, housing and care of laboratory animals. In: World animal sciences, Ruitenberg E.J., Peters P.W.J. (Editors). Elsevier science publishers.1986;pp:1-40.
- ژاله شجاعيان، سيد محمد مؤذني، اميرحسن زرناني، مقايسه توانايي سلول‌هاي دندريتيك طحال موش‌هاي حامله و غير حامله در تحريك آلوژنيك لنفوسيت‌هاي T، فصلنامه پزشكي باروري و ناباروري، سال 5 (1382)، شماره 1، صفحات 13-5.
- Erlebacher A. Why isn’t the fetus rejected? Current Opinion in Immunol.2001;13:590-593.
- Wolf-Levin R., Aoki K., Azuma T., Yagami Y., Okada H. Human pregnancy serum suppresses the proliferative response of lymphocytes to auto-logous PHA-activated T lymphoblasts. Am J Reprod Immunol.1996;35:63-69.
- Domingo G.D., Moreno A., Palomino P. The effect of human pregnancy serum on the synthesis and action of interleukin-1.J Reprod Imunol.1988; 13:17-30.
- Bartho J., Wegmann T.G. A progesterone-dependent immunomodulatory protein alters the Th1/ Th2 balance. J Reprod Immunol.1996; 31: 81-95.
- Malinowski A., Szpakowski M., Tchorzewski H., Zeman K., Kowalczyk T., Podciechowski L., Oszukowski P. Immunomodulating properties of sera in normal pregnant women. Ginekol Pol. 1992; 63:439-446.
- Manak R.C. Mitogenic responses of peri-pheral blood lymphocytes from pregnant and ovariectomized heifers and their modulation by serum. J Reprod Immunol.1982; 4:263-276.
- Luppi P. How immune mechanisms are affected by pregnancy. Vaccine.2003; 21:3352-3357.
- Tawfik O.W., Hunt J.S., Wood G.W. Impli-cation of prostaglandin E2 in soluble factor med-iated immune suppression by murine decidual cells. Am J Reprod Immunol Microbiol.1986; 12 (4):111-7.
- Lila N., Carpentier A., Amrein C., Khalil-Daher I., Dausset J., Carosella E.D.Implication of HLA-G molecule in heart graft acceptance. Lancet.2000;355(9221):2138.
- Hunt J.S., Jadhav L., Chu W., Geraghty D.E., Ober C. Soluble HLA-G circulates in maternal blood during pregnancy.Am J Obstet Gynecol. 2000;183(3):682-8.
- Allan D.S., Mc Michael A.G., Braud V.M. The ILT family of leukocyte receptors. Immuno-biology.2000;202(1):34-41. Review.
- Friec G., Laupeze B., Fardel O., Sebti Y., Pangault C., Guilloux V., Beauplet A., Fauchet R., Amiot L. Soluble HLA-G inhibits human dendritic cell-Triggered allogeneic T-cell prolife-ration without altering dendritic cell differentia-tion and maturation processes. Hum Immunol. 2003;64:752-761.
- Steinbrink K., Wolfl M., Jonuleit H., Knop J., Enk A.H. Induction of tolerance by IL-10-treated dendritic cells. J Immunol.1998;159:4772-4780.
- Kalinski P., Schuitemaker J.H., Hilkens C.M., Kapsenberg M.L. Prostaglandin E2 induces the final maturation of IL-12-deficient CD1a+ CD83+ DCS: The levels of IL-12 are determined during the final dendritic cell maturation and are resistant to further modulation. J Immunol. 1998; 61:2804-2809.
- Badet M.T., Bell S.C., Billington W.D. Imm-unoregulatory activity of supernatants from short-term cultures of mouse decidua tissue. J Reprod Fertil.1983;68:351-358.