- Ali Khosrowbeygi
- - Department of Clinical Biochemistry, Drug Applied Research Center, Tabriz University of Medical , Tabriz, Iran
- Nosratollah Zarghami
 - - Department of Clinical Biochemistry, Drug Applied Research Center, Tabriz University of Medical , Tabriz, Iran
- Jafar Nouroozzadeh
- - Clinical Biochemistry Department, Faculty of Medicine, Urmia Medical Sciences University, Urmia, Iran
- Maerefat Ghaffari
- - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Received: 4/1/2004 Accepted: 4/1/2004 - Publisher : Avicenna Research Institute |
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Abstract
Introduction: Although all leading causes of impaired sperm motility are not completely understood, poor sperm forward motility (asthenozoospermia) is considered to contribute to the infertility of a significant number of males. One of the major factors that can potentially cause asthenozoospermia is oxidative stress induced by reactive oxygen species (ROS). The aim of this study was to investigate lipid peroxidation by measuring a novel marker of lipid peroxide-tion, F2α-isoprostane, and malondialdehyde (MDA) in seminal plasma of normozoospermic vs. asthenozoospermic men, and their relationship with sperm motility. Materials and Methods: We designed a case-control study with a total subject of 30 males. After semen analysis subjects were determined to be either normozoospermic, as the control group (n=15), or asthenozoospermic, as the case group (n=15). Seminal plasma level of F2α-isoprostane was measured using a commercially available enzyme immunoassay (EIA) kit. The amount of MDA was determined by the thiobarbituric acid (TBA) assay. The Mann-Whitney test was used to compare results of the two groups. Coefficients of correlation between sperm quality parameters and the concentration of Isoprostan F2α and MDA were calculated using Spearman’s correlation analysis. Results: The mean level of F2α-isoprostane did not show a significant difference between the two groups (p>0.05). But, the difference of mean MDA between the two groups was statistic-ally significant (p<0.05 ). sperm motility was inversely correlated with both f2α-isoprostane and mda levels (p<0.05). conclusion: our results suggest that sperm membrane lipid peroxidation might not have a significant role in asthenozoospermia etiology, but further investigations are needed for confirmation.< pan>
Keywords:
Lipid peroxidation, Sperm motility, F2a-Isoprostane, Malondialdehyde, Seminal plasma, Asthenospermia, Normospermia To cite this article:References
- Hull M.G., Glazener C.M., Conway D.I., Fos-ter P.A., Hinton R.A., Coulson C., Lambert P.A., Watt E.M. Population study of causes, treat-ment and outcome of infertility. Br Med J.1995;291: 1693-97.
- Ollero M., Gil-Guzman E., Lopez M.C., Shar-ma R.K., Agarwal A., Larson K., Evenson D., Th-omas A.J. Jr., Alvarez J.G. Characterization of subsets of human spermatozoa at different stages of maturation: implicatin in the diagnosis and tre-atment of male infertility. Hum Reprod.2001;16: 1912-21.
- Pasqualotto F.F., Sharma .K., Nelson D.R., Thomas A.J. Jr., Agarwal. A. Relationship bet-ween oidative stress, semen characteristics, and clinical diagnosis in men undergoing infertility investigation. Fertil Steril.2000;73:459-64.
- Gagnon C., de Lamirande E. Male sterility and motility disorders, 1 Edition, published by Spring-er-Verlag, New York.1999;pp:37-44.
- Antoine L., Karine L., Jerome G., Anne-Marie P. Values of sperm thiobarbituric acid-reactive substance in fertile men. Clin Chim Acta. 2002; 325:113-15.
- Zorn B., Vidmar G., Meden-Vrtovec H. Semin-al reactive oxygen species as predictors of fertiliz-ation, embryo quality and pregnancy rates after conventional in vitro fertilization and intracytopl-asmic sperm injection.Int J Androl.2003;26:279-85.
- Gil-Guzman E., Ollero M., Lopez M.C., Shar-ma R.K., Alvarez J.G., Thmas A.J. Jr., Agarval A. Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation. Hum Reprod.2001;16:1922-30.
- Agarwal A., Saleh R.A. Role of oxidants in male infertility: rationale, significance, and treat-ment. Urol Clin North Am. 2002;29:817-27.
- Conquer J.A., Martin J.B., Tummon I., Watson L., Tekpetey F.Fatty acid analysis of blood serum, seminal plasma, and spermatozoa of normozoosp-ermic vs. asthenozoospermic males. Lipids.1999; 34:793-9.
- Koksal I.T., Usta M., Orhan I., Abbasoglu S., Kadioglu A. Potential role of reactive oxygen species on testicular pathology associated with infertility. Asian J Androl. 2003;5:95-9.
- Segnini A., Camejo M.I., Proverbio F. Chlam-ydia trachomatis and sperm lipid peroxidation in infertile men. Asian J Androl. 2003;5:47-9.
- Sanocka D., Kurpisz M. Reactive oxygen species and sperm cells. Reprod Biol Endocrinol. 2004;2:12-19.
- Agarwal A., Saleh R.A., Bedaiwy M.A. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril.2003;79:829-43.
- Armstrong J.S., Bivalacqua T.J., Chamulitrat W., Sikka S., Hellstrom W.J. A comparison of the NADPH oxidase in human sperm and white blood cells. Int J Androl.2002;25:223-9.
- Meagher E.A., FitzGerald G.A. Indices of lipid peroxidation In Vivo: strengths and limit-ations. Free Radic Biol Med.2000;28:1745-50.
- Gomez E., Irvine D.S., Aitken R.J. Evaluation of a spectrophotometric assay for the measure-ment of malondialdehyde and 4-hydroxyalkenals in human spermatozoa: relationships with semen quality and sperm function. Int J Androl. 1998;21: 81-94.
- Lawson J.A., Rokach J., FitzGerald G.A. Isoprostanes: formation, analysis and use as indices of lipid peroxidation In Vivo. J Biol Chem. 1999; 274:24441-4.
- Roberts L.J., Morrow J.D. Measurement of F(2)-isoprostanes as an index of oxidative stress In Vivo. Free Radic Biol Med. 2000;28:505-13.
- Pratico D., Lawson J.A., Rokach J., Fitz Gerald G.A. The isoprostanes in biology and medicine. Trends Endocrinol Metab.2001;12:243-7.
- Ichikawa T., Oeda T., Ohmori H., Schill W.B. Reactive oxygen species influence the acrosome reaction but not acrosin activity in human sperma-tozoa. Int J Androl. 1999;22:37-42.
- Rhemrev J.P., Vermeiden J.P., Haenen G.R., De Bruijne J.J., Rekers-Mombarg L.T., Bast A. Progressively motile human spermatozoa are well protected against in vitro lipid peroxidation Impo-sed by induced oxidative stress. Andrologia.2001; 33:151-8.
- Dandekar S.P., Nadkarni G.D., Kulkarni V.S., Punekar S. Lipid peroxidation and antioxidant enzymes in male infertility. J Postgrad Med. 2002;48:186-89.
- Ozgocmen S., Sogut S., Fadillioglu E., Ardic-oglu A., Ardicoglu O. Antioxidant status and lipid peroxidation in seminal plasma and spermatozoa of patients with ankylosing spondylitis. Rheumat-ology.2003;42:805-7.
- Suleiman S.A., Ali M.E., Zaki Z.M., el-Malik E.M., Nasr M.A. Lipid peroxidation and human sperm motility: protective role of vitamin E. J Androl. 1996;17:530-37.
- Fraczek M., Szkutnik D., Sanocka D., Kurpisz M. Peroxidation components of sperm lipid membranes in male infertility. Ginekologia Polska. 2001;72:73-79.
- Nakamura H., Kimura T., Nakajima A., Shimoya K., Takemura M., Hashimoto K., Isaka S., Azuma C., Koyama M., Murata Y. Detection of oxidative stress in seminal plasma and fraction-ated sperm from subfertile male patients. Eur J Obstet Gynecol Reprod Biol. 2002;105:155-60.
- Keskes-Ammar L., Feki-Chakroun N., Rebai T., Sahnoun Z., Ghozzi H., Hammami S., Zghal K., Fki H., Damak J., Bahloul A. Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl. 2003;49:83-94.
- World Health Organization: WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interactin. Cambridge University Press, Cambridge, UK, 1999.
- Nourooz-Zadeh J., Tajaddini-Sarmadi J., Mc Carthy S., Betteridge D.J., Wolff S.P. Elevated levels of authentic plasma hydroperoxides in NIDDM. Diabetes.1995;44:1054-8.
- Roberts L.J. 2nd, Morrow J.D. Products of the isoprostane pathway: unique bioactive compounds and markers of lipid peroxidation. Cell Mol Life Sci. 2002;59:808-20.
- Nonaka-Sarukawa M., Yamamoto K., Aoki H., Takano H., Katsuki T., Ikeda U., Shimada K. Increased urinary 15-F2t-isoprostane concentra-tions in patients with non-ischaemic congestive heart failure: a marker of oxidative stress. Heart. 2003;89:871-4.