JRI 
Vol. 23, Issue 3, / July-September 2022
(Case Report, pages 224-227)
PMID: 36415490 (PubMed) - PMCID: PMC9666591

Roya Padmehr
- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Saeid Arasteh
- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Soheila Aminimoghaddam
- Department of Gynecology Oncology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Ali Rahbari
- Assistance Professor of Pathology, Arak University of Medical Sciences, Tehran, Iran
Mehrdad Bohloli
- Department of Surgery, Jam General Hospital, Tehran, Iran
Seyed Mohammad Mir Eskandari
- Assistance Professor of Anesthesiology and Intensive Care, Tehran University of Medical Sciences, Tehran, Iran
Hamid Mohabbat Dar
- Shahid Beheshti University of Medical Sciences, Tehran, Iran
Morvarid Ahmad Beigi Corresponding Author
- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Negin Talebi Biderouni
- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Received: 1/25/2022 Accepted: 3/2/2022 - Publisher : Avicenna Research Institute

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Abstract

Background: Perivascular Epithelioid Cell Tumors (PEComas) are rare mesenchymal tumors originating from perivascular epithelioid cells. The second common affected organ is uterine. Most of PEComas are benign and patients have good prognosis. At the present time, surgery is the main treatment and adjuvant chemotherapy is used in malignant cases, although the best diagnostic and management method is yet to be discovered considering the rarity of this neoplasm.
Case Presentation: The patient was a 53 year old lady with a history of two vaginal deliveries and no previous surgery. She had severe pelvic pain and underwent MRI with the primary impression of sarcoma. In MRI, she had a 7 cm mass in lower segment of uterus. The patient underwent laparoscopic hysterectomy, bilateral oophorectomy, lymphadenectomy, and omental biopsy in Jam Hospital. Pathologic report of the patient revealed malignant PEComa without lymph node and omentum involvement.
Conclusion: Diagnosis of PEComa before surgery is difficult and its differential diagnoses form uterine leiomyoma or leiomyosarcoma. Final diagnosis can be made after surgical biopsy and immunohistochemistry evaluation. Surgery is still the main treatment and adjuvant therapy is used in high risk patients.



Keywords: Case report, Pathology, PEComa, Perivascular epithelioid cell tumor


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Full Text

Introduction
Perivascular Epithelioid Cell Tumors (PEComas) are rare mesenchymal tumors first reported in 1995. The term "PEComa" was used to describe tumors originated from perivascular epithelioid cells in 1996 (1). PEComas are a family of mesenchymal tumors including angiomyolipomas, clear cell tumors of the lung, and lymphangioleimyomas. Most of these rare tumors are benign. Besides peritoneum, the second common organ affected by the tumor is uterus (2). Up to now, about 100 patients with PEComas are reported among whom one third had uterine involvement. As affected cases of this disease were not reported, standard diagnostic and management procedure have not been described yet. In this case report, a patient with uterine PEComa is reported and described.

Case presentation
The patient was a 53 year old lady, who reached menopause 3 years ago, with a history of 2 vaginal deliveries. She had severe pelvic pain and abnormal uterine bleeding and underwent diagnostic curettage before visiting us. The report was normal with atrophic endometrium. Sonographic evaluation revealed a lobulated hypoechoic mass, 67x46x64 mm in size, from posterior uterine wall towards cervix. Gynaecologic evaluation revealed a mass in left side of uterus and MRI report was suggestive of a uterine sarcoma.
The patient underwent bilateral oophorectomy and hysterectomy. A 5 mm and a 12 mm trocar were used to enter abdominal cavity. A mass behind cervix and frozen pelvis were observed. An enlarged uterine with a bulging cervix, adhesion of ovaries to pelvic floor, and intestinal adhesion to cervix and left adnexa were observed. Uterine with the mass in cervix was separated intactly and removed from vagina (manipulator was not used due to probability of malignancy). The sample was sent to frozen section and in frozen section, using cryotomy and touch smear, a tumor consisting of monomorphic cells with moderate atypia and necrosis sites was observed. Mesenchymal tumor in favor of cervical sarcoma was reported. Left parametrium was thoroughly involved compressing left urethra. Right parametrium had lower involvement. Bilateral double J was inserted. The mass had sarcomatous pattern. Left parametrium was completely removed. An omental biopsy was performed. Then lymph nodes of both obturator and iliac region, left and right sides, were removed completely. An old thrombotic vein in pelvic floor and left side was observed and was not touched due to its creation of thrombosis in her last pregnancy. Samples were sent to pathology laboratory for cytological and immunohistochemistry evaluation.
In pathologic sample, a round soft mass in uterus wall, 7 cm in diameter, was observed and cervical mucosa was normal. Tissue samples were fixed by formalin 10% and colored with hematoxylin-eosin. A tumor consisting of monomorphic cells in regular nests and hypervascular background including delicate microvasculature separating cellular nests was observed. In larger view, multidimensional cells with clear cytoplasm and same nucleus with low to moderate atypia were observed. Cells were around vessels. Cells were colored using immunohistochemistry with HMB45, vimentin, and Melan A makers. P63, S100, cytokeratin, desmin, h-caldesmon were negative and differential diagnosis for the patient was PEComas (Figures 1-6).
Pathology lab report revealed malignant PEComa without involvement of peritoneum, pelvis, parametriums, lymph nodes, and omentum. The patient was discharged after two days with a good general health and was referred to the radiotherapist. As there was no pelvic involvement, NEORAPA (antineoplastic agent) and Rapamune were prescribed for the patient. The patient is followed regularly.

Discussion
Up to now, about 100 patients with PEComa of uterine are reported. Mean age of patients was about 40 years (4) though it can be observed in all age groups. Hormones may play role in its pathogenesis in women (5, 6). Malignancy potential of PEComa depends on histopharmacology of prima-ry tumor and not its origin. PEComas are more likely to affect retroperitoneum, kindies, and female reproductive system; yet, it occurs in other body parts as well. Cervix is rarely involved (7, 8). Differential diagnosis of PEComas include all types of benign and malignant tumors. PEComas may be mistaken for carcinomas such as clear cell tumor especially if the patient has involvement of cervix or bladder (4, 9). Pathogenesis of PEComa is still unclear and it is hypothesized that they are related to complex tuberous sclerosis (9).
Clinical presentation, physical examination of pelvis, and pelvis ultrasound are not specific and can be mistaken for benign tumors or uterine fibroids or other malignancies. This non-specificity makes difficulties in diagnosis and management of PEComa and delays treatment of some patients (10-12). Furthermore, patients with malignancy have high survival rates without adjutant treatment. Chemotherapy regimen for malignant PEComa of reproductive system is not well defined and need more studies. Currently, the role of radiotherapy is not clear enough. Histological analysis of malignant PEComa reveals high mitotic index and multiple necrosis. Necrosis is related to tolerance threshold of chemotherapy and high angiogenesis shows high sensitivity of cells to radiation; therefore, these two statements support the role of radiotherapy for malignant PEComa (4). Systemic chemotherapy regimen without surgery is not an effective treatment. Thus, adjuvant chemotherapy has not an effective role in prognosis of patients with malignant PEComa and surgery is still the main treatment. Most malignant PEComas has good survival without recurrence or metastasis (13). Most patients with PEComa undergo bilateral oophorectomy and total hysterectomy based on their age (14). When a simple surgery may be adequate for both benign and malignant PEComa, adjuvant therapy after surgery should be provided for patients in spite of weak efficacy of radiotherapy and chemotherapy (15). Different studies have indicated increase in survival rate after surgery by using combination of chemotherapy and radiotherapy (16).

Conclusion
Uterine PEComa is a rare disease and the main diagnosis is made after pathologic assessment of patient’s sample following surgery and immunohistochemistry. Prognosis of the disease is depending on whether the tumor is benign or malignant. The first treatment for patients is surgery and in malignant cases, adjuvant therapy is needed for reducing the risk of recurrence and metastasis.

Conflict of Interest
The authors declare that they have no conflict of interest.




Figures, Charts, Tables

High Resolution
Figure 1. Monomorphic cells in regular nests and a hyper-vascular background including microvasculature separating cellular nests

Figure 1. Monomorphic cells in regular nests and a hyper-vascular background including microvasculature separating cellular nests



High Resolution
Figure 2. Larger view of multidimensional cells with clear cytoplasm, same-sized nucleus, and low atypia in dense groups

Figure 2. Larger view of multidimensional cells with clear cytoplasm, same-sized nucleus, and low atypia in dense groups



High Resolution
Figure 3. Neoplastic cells around vessels

Figure 3. Neoplastic cells around vessels



High Resolution
Figure 4. Fibrotic bands with lobular pattern in tumor

Figure 4. Fibrotic bands with lobular pattern in tumor



High Resolution
Figure 5. Immunohistochemistry with HBM45 marker

Figure 5. Immunohistochemistry with HBM45 marker



High Resolution
Figure 6. Immunohistochemistry with Melan A marker

Figure 6. Immunohistochemistry with Melan A marker



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